Expression, purification and identification of Pla a1 in a codon-optimized Platanus pollen allergen
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چکیده
منابع مشابه
Expression, purification and identification of Pla a1 in a codon-optimized Platanus pollen allergen.
The present study aimed to express, purify and identify the major allergen gene, Pla a1, in Platanus pollen. According to previous studies, the major gene sequences of the Pla a1 allergen were obtained and codon optimization and synthesis of the genome were performed using DNAStar software. Following binding of the target gene fragment and the pET-44a vector, the JM109 cells were transfected to...
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ژورنال
عنوان ژورنال: Molecular Medicine Reports
سال: 2015
ISSN: 1791-2997,1791-3004
DOI: 10.3892/mmr.2015.3652